. To optimize manufacturing and supply situations, genes ≤5 kb in size are shipped in a “best-match” vector (Table 1), by using a preference between ampicillin or kanamycin choice markers. We also supply vectors that were optimized to be used with Golden Gate Assembly; they will not possess the most frequently employed Form IIS restriction si

Developing on this function and leveraging our genetically built-in FailSafe (FS) suicide system36,37, we also clearly show that the dormant subcutaneous tissues generated from these engineered mouse embryonic stem cells (mESCs) retain transgene overexpression and guard unmodified cells or tissue injected in the modified tissue. In doing this, we p